gene isolation pdf
GENE ISOLATION 87 entire library several times. 0000004152 00000 n 0000076357 00000 n There are three main methods for obtaining genes 1. 0000006287 00000 n �;a���J�X�0�a������F�[�)1p=*�l �"'���1p}���:��2�� � xE� endstream endobj 104 0 obj 274 endobj 58 0 obj << /Type /Page /MediaBox [ 0 0 439.19934 669.59912 ] /Parent 50 0 R /CAPT_Info << /R [ 0 5580 0 3660 ] /S [ 0 2790 0 1830 ] /Rz [ 300 300 300 300 0 0 ] /SK (0085)>> /Contents [ 64 0 R 68 0 R 72 0 R 74 0 R 76 0 R 80 0 R 93 0 R 95 0 R ] /Resources << /XObject << /Im0 97 0 R >> /ExtGState << /GS0 98 0 R /GS1 99 0 R /GS2 100 0 R /GS3 101 0 R /GS4 102 0 R >> /ColorSpace << /CS0 62 0 R /CS1 61 0 R >> /Font << /TT0 60 0 R /TT1 65 0 R /TT2 69 0 R /TT3 77 0 R /TT4 81 0 R /T1_0 88 0 R /TT5 91 0 R /T1_1 86 0 R >> /ProcSet [ /PDF /Text /ImageB ] >> /Rotate 0 /CropBox [ 0 0 439.19934 669.59912 ] /LastModified (D:20020613100216) /Thumb 34 0 R >> endobj 59 0 obj << /Type /FontDescriptor /FontName /TimesNewRomanPSMT /FontBBox [ -250 -250 1009 1000 ] /Flags 34 /CapHeight 712 /Ascent 891 /Descent 216 /StemV 73 /ItalicAngle 0 /XHeight 498 /Leading 42 /AvgWidth 401 /MaxWidth 1175 >> endobj 60 0 obj << /Type /Font /Subtype /TrueType /BaseFont /TimesNewRomanPSMT /FirstChar 0 /LastChar 255 /Encoding /WinAnsiEncoding /FontDescriptor 59 0 R /Widths [ 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 250 333 408 500 500 833 778 180 333 333 500 564 250 333 250 278 500 500 500 500 500 500 500 500 500 500 278 278 564 564 564 444 921 722 667 667 722 611 556 722 722 333 389 722 611 889 722 722 556 722 667 556 611 722 722 944 722 722 611 333 278 333 469 500 333 444 500 444 500 444 333 500 500 278 278 500 278 778 500 500 500 500 333 389 278 500 500 722 500 500 444 480 200 480 541 778 500 778 333 500 444 1000 500 500 333 1000 556 333 889 778 611 778 778 333 333 444 444 350 500 1000 333 980 389 333 722 778 444 722 250 333 500 500 500 500 200 500 333 760 276 500 564 333 760 500 400 549 300 300 333 576 453 250 333 300 310 500 750 750 750 444 722 722 722 722 722 722 889 667 611 611 611 611 333 333 333 333 722 722 722 722 722 722 722 564 722 722 722 722 722 722 556 500 444 444 444 444 444 444 667 444 444 444 444 444 278 278 278 278 500 500 500 500 500 500 500 549 500 500 500 500 500 500 500 500 ] >> endobj 61 0 obj /DeviceGray endobj 62 0 obj /DeviceRGB endobj 63 0 obj 746 endobj 64 0 obj << /Filter /FlateDecode /Length 63 0 R >> stream 0000019049 00000 n Some ��:��������P3N trailer << /Size 105 /Info 51 0 R /Root 56 0 R /Prev 721095 /ID[<14e0095756d62ec684ac56ab6690c9c2>] >> startxref 0 %%EOF 56 0 obj << /Type /Catalog /Pages 50 0 R /CAPT_Info << /L [ (English US)(English UK)] /D [ [ ] [ (Default)()] ] >> /FICL:Enfocus 52 0 R /PageLayout /SinglePage /OpenAction 57 0 R /Metadata 49 0 R >> endobj 57 0 obj << /S /GoTo /D [ 58 0 R /Fit ] >> endobj 103 0 obj << /S 155 /T 292 /Filter /FlateDecode /Length 104 0 R >> stream The basic steps in a gene cloning experiment are: i. Gene isolation. 0000012689 00000 n �&. Isolation of gene … 0000016970 00000 n gene isolation strategies Since the first reported isolation of a B-glucosidase gene from Escherichia adecarbox&ta in 1981 (1) and a Cellulomonas jimi exoglucanase gene in … Figure 3: Applications of CRISPR-associated protein 9 (Cas9) in gene editing, gene regulation, epigenome editing, and genomic imaging. 0000001348 00000 n 0000010404 00000 n 0000076503 00000 n H��TM��0��+��J]�`��J�=l�v�^�$��S�&ʿ�lL��H��7ｙ���� �T��ů�%��[�%���I~$t��� $���Ucs��Lkl���65�&��V1���0H9� +r|V]�)��կ�S�Ykx�h�8E���m��e0��T�����3"9+}�c����K\��z`������(|� 0000009620 00000 n The plasmid carried by this transformant is then recovered from the transformant and the insert fragment characterized. Amgen 22,635 views. 0000008798 00000 n stream gene isolation strategies Since the first reported isolation of a B-glucosidase gene from Escherichia adecarbox&ta in 1981 (1) and a Cellulomonas jimi exoglucanase gene in 1982 (103) there … �u7�F\GegT�1�k�m�����5"1֡���K�G�RGa��8 0000004230 00000 n �������IvL���=�Q��n���HS�]F�>�A������-.��'�m!�q�3�� !������XO����㝪Gc��M��#8��ʇ��B�� ���� endstream endobj 65 0 obj << /Type /Font /Subtype /TrueType /BaseFont /TimesNewRomanPS-BoldMT /FirstChar 0 /LastChar 255 /Encoding /WinAnsiEncoding /FontDescriptor 66 0 R /Widths [ 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 778 250 333 555 500 500 1000 833 278 333 333 500 570 250 333 250 278 500 500 500 500 500 500 500 500 500 500 333 333 570 570 570 500 930 722 667 722 722 667 611 778 778 389 500 778 667 944 722 778 611 778 722 556 667 722 722 1000 722 722 667 333 278 333 581 500 333 500 556 444 556 444 333 500 556 278 333 556 278 833 556 500 556 556 444 389 333 556 500 722 500 500 444 394 220 394 520 778 500 778 333 500 500 1000 500 500 333 1000 556 333 1000 778 667 778 778 333 333 500 500 350 500 1000 333 1000 389 333 722 778 444 722 250 333 500 500 500 500 220 500 333 747 300 500 570 333 747 500 400 549 300 300 333 576 540 250 333 300 330 500 750 750 750 500 722 722 722 722 722 722 1000 722 667 667 667 667 389 389 389 389 722 722 778 778 778 778 778 570 778 722 722 722 722 722 611 556 500 500 500 500 500 500 722 444 444 444 444 444 278 278 278 278 500 556 500 500 500 500 500 549 500 556 556 556 556 500 556 500 ] >> endobj 66 0 obj << /Type /FontDescriptor /FontName /TimesNewRomanPS-BoldMT /FontBBox [ -250 -250 1089 1000 ] /Flags 34 /CapHeight 712 /Ascent 891 /Descent 216 /StemV 136 /ItalicAngle 0 /XHeight 498 /Leading 42 /AvgWidth 427 /MaxWidth 1273 >> endobj 67 0 obj 657 endobj 68 0 obj << /Filter /FlateDecode /Length 67 0 R >> stream "����n�p�?�X��)s(Y�*���A���)4)�Tsd��F�(��T8��R������>p4�F"��$ǵn(kI� $�Sb�F�������嬷�%�Q;���F�6��������uw�����p�u�C�.����Fn �������̸S��l&L���}'HA���I��Mn��ԟn�Q�[ׂɼ�"*�2�ͼ���[��I�+0��3��0v��f讌����b���y�p}{�=^�x��8�}n������6�{Ӈ��HZd�Y�~���]N�4�3B]z�S�K�,XW'մ�=N*�Ch��3�����A����=�܌�{V%a��xP�rsJ�aT��"���Q/�j�6'm� , During DNA replication it joins Okazaki fragments to create a continuous strand, and in cloning, it is used to join the various DNA fragments with the vector. 0000006544 00000 n Is|�r�p�~��� iii. Gene Isolation and Manipulation 4. 0000076710 00000 n Gene Cloning 3. 0000019794 00000 n Vector preparation: bacterial plasmid is cut open using the same restriction enzyme 3. 0000004209 00000 n Using the PCR 3. 0000016099 00000 n 0000018274 00000 n 0000018253 00000 n Chapter 10: “Gene Isolation and Manipulation” Sections 1-4, 6 2. 0000076221 00000 n Methods of Gene Isolation Promila Sheoran Ph.D. Biotechnology GJU S&T Hisar 2. It involves a lossless complexity reduction … Isolation and Gene Expression Analysis of Arabidopsis thaliana Mutants With Constitutive Expression of ATL2, an Early Elicitor-Response RING-H2 Zinc-Finger Gene This article is dedicated to the memory of the late Gilberto Mosqueda Cano. Identification and Isolation of the Gene of Interest - Duration: 1:17. 0000001985 00000 n ‘Gene Cloning’ is a method of isolating a specific region of DNA and producing millions of identical copies of the DNA (gene) within a microbial cell culture. 1:17. H�tU��� ��������Q�M�.�B��Ŏ�!�g�>? 0000015075 00000 n Request PDF | How to isolate a gene, A general overview | Introduction The basic purpose of gene isolation is to integrate it with the host cell to get the desired product usually desired protein. << /Length 1 0 R /Filter /FlateDecode >> 0000002007 00000 n 0000015600 00000 n 55 0 obj << /Linearized 1 /O 58 /H [ 1617 390 ] /L 722323 /E 77399 /N 6 /T 721105 >> endobj xref 55 50 0000000016 00000 n 0000014663 00000 n
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