Recombinant DNA technology also referred to as molecular cloning is similar to polymerase chain reaction ( PCR ) in that it permits the replication of a specific DNA sequence. The recombinant DNA technology emerged with the discovery of restriction enzymes in the year 1968 by Swiss microbiologist Werner Arber, Inserting the desired gene into the genome of the host is not as easy as it sounds. Recombinant DNA technology is popularly known as genetic engineering. Small molecular weight compounds and macromolecules, which are naturally present in very low amounts, … This technology is currently used in both basic and research studies. The fundamental difference between the two methods is that molecular cloning involves replication of the DNA in a living microorganism, while PCR replicates DNA in an in vitro solution, free of living cells. This process has been responsible for fueling many advances related to modern molecular biology. Some of the recent advances made possible by recombinant DNA technology are: Genetic Engineering. 2014.00172. 5. doi: 10.3389/fmicb. It looks at some of the enzymes and other fundamental tools for working with DNA and cells that are used to manipulate and analyze DNA, including determining its sequence; to clone DNA; and to analyze proteins. Recombinant DNA technology engineers microbial cells for producing foreign proteins, and its success solely depends on the precise reading of equivalent genes made with the help of bacterial cell machinery. This chapter looks at some of the tools scientists use in carrying out research of recombinant DNA. Recombinant DNA technology has opened new horizons in many fields of science such as medicine, pharmacy, biology, nutrition, biochemistry, microbiology, genetics, immunology, agriculture and environmental engineering. Recombinant DNA technology, apart from being an important tool of scientific research, has also played a vital role in the diagnosis and treatment of various diseases, especially those belonging to genetic disorders. Learn recombinant dna technology microbiology with free interactive flashcards. Frontiers in Microbiology. Choose from 500 different sets of recombinant dna technology microbiology flashcards on Quizlet.

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